Composite
Part:BBa_K1039014:Design
Designed by: Jama Hagi-Yusuf Group: iGEM13_Waterloo (2013-09-12)
PhiC31 Intgrase and Recombination Directionality Factor Expression Cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 112
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2561
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1726
Illegal SapI site found at 2227
Illegal SapI site found at 2449
Illegal SapI site found at 2575
Illegal SapI.rc site found at 1417
Illegal SapI.rc site found at 1475
Illegal SapI.rc site found at 1580
Design Notes
The following mutation was made on PhiC31 integrase Base 51 is changed from T to C, silent mutation. The sequence was synthesized by a third party company.
Source
Biobricks to make this part were synthesized by BioBasic Inc or from the iGEM 2013 distribution.