Composite

Part:BBa_K1039014:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-12)


PhiC31 Intgrase and Recombination Directionality Factor Expression Cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1726
    Illegal SapI site found at 2227
    Illegal SapI site found at 2449
    Illegal SapI site found at 2575
    Illegal SapI.rc site found at 1417
    Illegal SapI.rc site found at 1475
    Illegal SapI.rc site found at 1580


Design Notes

The following mutation was made on PhiC31 integrase Base 51 is changed from T to C, silent mutation. The sequence was synthesized by a third party company.


Source

Biobricks to make this part were synthesized by BioBasic Inc or from the iGEM 2013 distribution.

References